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7.7 colocalization plugin  (MetaMorph Inc)

 
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    Structured Review

    MetaMorph Inc 7.7 colocalization plugin
    7.7 Colocalization Plugin, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7.7 colocalization plugin/product/MetaMorph Inc
    Average 90 stars, based on 1 article reviews
    7.7 colocalization plugin - by Bioz Stars, 2026-03
    90/100 stars

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    Autophagy is triggered and mediates the secretion of IL-1β upon stimulation of wear particles. (A, B) WB and immunofluorescence results of autophagy-related proteins in RAW264.7 cells challenged with different wear particles (scale bar: 20 μm). (C) Quantification of LC3-II level in WB. (D) Quantification of LC3 immunofluorescence intensity. (E) Concentrations of IL-1β and pro-IL-1β in culture supernatants of RAW264.7 cells stimulated with wear particles for 12 h in the absence or presence of 3-methyladenine (3-MA) (5 mM). (F) Immunofluorescence staining on RAW264.7 cells and 3D reconstruction for IL-1β (red), LC3 (green), and DAPI (blue) (scale bar: 5 μm). Yellow spots indicate co-localization. (G) 3D co-localization analysis was conducted using the IMARIS <t>coloc</t> <t>plugin.</t> (H) Co-localization analysis of red channel (IL-1β) and green channel (LC3) conducted by FIJI software. (I) Colocalization line tracing analysis from images in (F). Gray arrows indicate the region of red-green overlap. (J) Pearson's colocalization coefficient for IL-1β and LC3. Sample size: n = 3 per group. All experiments were performed with n = 3 independent biological replicates. (ns indicates P > 0.05, *** indicates P < 0.001, and **** indicates P < 0.0001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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    Image Search Results


    Autophagy is triggered and mediates the secretion of IL-1β upon stimulation of wear particles. (A, B) WB and immunofluorescence results of autophagy-related proteins in RAW264.7 cells challenged with different wear particles (scale bar: 20 μm). (C) Quantification of LC3-II level in WB. (D) Quantification of LC3 immunofluorescence intensity. (E) Concentrations of IL-1β and pro-IL-1β in culture supernatants of RAW264.7 cells stimulated with wear particles for 12 h in the absence or presence of 3-methyladenine (3-MA) (5 mM). (F) Immunofluorescence staining on RAW264.7 cells and 3D reconstruction for IL-1β (red), LC3 (green), and DAPI (blue) (scale bar: 5 μm). Yellow spots indicate co-localization. (G) 3D co-localization analysis was conducted using the IMARIS coloc plugin. (H) Co-localization analysis of red channel (IL-1β) and green channel (LC3) conducted by FIJI software. (I) Colocalization line tracing analysis from images in (F). Gray arrows indicate the region of red-green overlap. (J) Pearson's colocalization coefficient for IL-1β and LC3. Sample size: n = 3 per group. All experiments were performed with n = 3 independent biological replicates. (ns indicates P > 0.05, *** indicates P < 0.001, and **** indicates P < 0.0001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Nanoscale ZnO doping in prosthetic polymers mitigate wear particle-induced inflammation and osteolysis through inhibiting macrophage secretory autophagy

    doi: 10.1016/j.mtbio.2024.101225

    Figure Lengend Snippet: Autophagy is triggered and mediates the secretion of IL-1β upon stimulation of wear particles. (A, B) WB and immunofluorescence results of autophagy-related proteins in RAW264.7 cells challenged with different wear particles (scale bar: 20 μm). (C) Quantification of LC3-II level in WB. (D) Quantification of LC3 immunofluorescence intensity. (E) Concentrations of IL-1β and pro-IL-1β in culture supernatants of RAW264.7 cells stimulated with wear particles for 12 h in the absence or presence of 3-methyladenine (3-MA) (5 mM). (F) Immunofluorescence staining on RAW264.7 cells and 3D reconstruction for IL-1β (red), LC3 (green), and DAPI (blue) (scale bar: 5 μm). Yellow spots indicate co-localization. (G) 3D co-localization analysis was conducted using the IMARIS coloc plugin. (H) Co-localization analysis of red channel (IL-1β) and green channel (LC3) conducted by FIJI software. (I) Colocalization line tracing analysis from images in (F). Gray arrows indicate the region of red-green overlap. (J) Pearson's colocalization coefficient for IL-1β and LC3. Sample size: n = 3 per group. All experiments were performed with n = 3 independent biological replicates. (ns indicates P > 0.05, *** indicates P < 0.001, and **** indicates P < 0.0001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Yellow spots indicate co-localization. (B) 3D co-localization analysis conducted by IMARIS coloc plugin. (C) Co-localization analysis of red channel (TRIM16) and green channel (LC3) conducted by FIJI software. (D) Colocalization line tracing analysis from images in (A).

    Techniques: Immunofluorescence, Staining, Software

    Sec22b and TRIM16 are involved in autophagy-mediated IL-1β secretion. (A) Immunofluorescence staining on RAW264.7 cells and 3D reconstruction for TRIM16 (red), LC3 (green), and DAPI (blue) (scale bar: 5 μm). Yellow spots indicate co-localization. (B) 3D co-localization analysis conducted by IMARIS coloc plugin. (C) Co-localization analysis of red channel (TRIM16) and green channel (LC3) conducted by FIJI software. (D) Colocalization line tracing analysis from images in (A). Gray arrows indicate the region of red-green overlap. (E) Pearson's colocalization coefficient for TRIM16 and LC3. (F) Immunofluorescence staining and 3D reconstruction for Sec22b (purple), LC3 (green), and DAPI (blue) (scale bar: 5 μm). White spots indicate co-localization. (G) 3D co-localization analysis conducted by IMARIS coloc plugin. (H) Co-localization analysis of purple channel (TRIM16) and green channel (LC3) conducted by FIJI software. (I) Colocalization line tracing analysis from images in (F). Gray arrows indicate the region of purple-green overlap. (J) Pearson's colocalization coefficient for Sec22b and LC3. Sample size: n = 3 per group. All experiments were performed with n = 3 independent biological replicates. (*** indicates P < 0.001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Nanoscale ZnO doping in prosthetic polymers mitigate wear particle-induced inflammation and osteolysis through inhibiting macrophage secretory autophagy

    doi: 10.1016/j.mtbio.2024.101225

    Figure Lengend Snippet: Sec22b and TRIM16 are involved in autophagy-mediated IL-1β secretion. (A) Immunofluorescence staining on RAW264.7 cells and 3D reconstruction for TRIM16 (red), LC3 (green), and DAPI (blue) (scale bar: 5 μm). Yellow spots indicate co-localization. (B) 3D co-localization analysis conducted by IMARIS coloc plugin. (C) Co-localization analysis of red channel (TRIM16) and green channel (LC3) conducted by FIJI software. (D) Colocalization line tracing analysis from images in (A). Gray arrows indicate the region of red-green overlap. (E) Pearson's colocalization coefficient for TRIM16 and LC3. (F) Immunofluorescence staining and 3D reconstruction for Sec22b (purple), LC3 (green), and DAPI (blue) (scale bar: 5 μm). White spots indicate co-localization. (G) 3D co-localization analysis conducted by IMARIS coloc plugin. (H) Co-localization analysis of purple channel (TRIM16) and green channel (LC3) conducted by FIJI software. (I) Colocalization line tracing analysis from images in (F). Gray arrows indicate the region of purple-green overlap. (J) Pearson's colocalization coefficient for Sec22b and LC3. Sample size: n = 3 per group. All experiments were performed with n = 3 independent biological replicates. (*** indicates P < 0.001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Yellow spots indicate co-localization. (B) 3D co-localization analysis conducted by IMARIS coloc plugin. (C) Co-localization analysis of red channel (TRIM16) and green channel (LC3) conducted by FIJI software. (D) Colocalization line tracing analysis from images in (A).

    Techniques: Immunofluorescence, Staining, Software